HPV typing

HPV genital wart virus, also known as human life converts nightmare. More than 100 types of HPV virus in the 5-6 range, especially prevalent in humans, warts while HPV 6 and 11 of more genera, 16 genera and 18 cause cancer and warts as well as doing. HPV virus, HIV (AIDS), then both men and women, the most common disease transmitted by sexual contact.

According to research in the United States between the ages of 15 and 49 percent of women and men 10 to 20 had HPV infection. showing the largest increase in the last ten years, this virus, now known to reach dangerous levels is not done.

Considered the most effective factor in the formation of cervical HPV virus is transmitted sexually. When the cells moved into the body, a person's immune system is weak fall times (stress, insomnia, eating disorders, etc.), even after many years of infection in people undergoing major role in the development of cervical cancer alevlenerek. For this reason, early detection of HPV infection is very important. . Today, the risk of HPV in diagnosis and determining the genetic laboratories is gaining great importance to studies made by the HPV-DNA. This test is also called HPV test.

Diagnosis of HPV are being How?

_ Pap Smear

_ HPV DNA genotyping (PCR), HPV testing

_ Biopsy: Koilositoz, piknotik nucleus, akanthosis, multinükleasyon ...

HPV Test, HPV-DNA PCR testing What is it?

Virus genetic material (DNA) is an analysis based on detection of the presence of high-risk HPV CIN2 / 3 + is considered to be more sensitive than cytology in the diagnosis.

In 2003, the FDA (American Food and Drug Administration) 30 women over the age of HPV-DNA analysis of the cancer screening, HPV test, along with the simultaneous use of the Pap smear test has confirmed. However, cervical cancer research in the literature used as a primary screening test is more accurate and in time began to run articles only being used as a screening test. Due to technical difficulties in evaluating the cervical smear test has the problem of reproducibility has been reported even in optimal conditions.

High-risk oncogenic virus genotypes (HPV-DNA PCR), the most important reason for the development of CIN and cervical cancer (97%) understood that. In cases of cervical cancer screening tests must be abnormal cytology with HPV DNA testing and genotyping kesinleştirilmesi the exact diagnosis, prognosis and treatment of lead on the show. This approach prevents patients without invasive surgical procedures. Cytology alone is applied and sensitivity of smear tests for cervical cancer prevention studies, being limited to constitute a significant problem. Microscopic analysis of cellular changes in Pap smear and HPV is able to detect the presence of proving insufficient. HPV-DNA PCR technique (HPV test) is applied at the low level of HPV positivity yakalanabilmekte and latent, insidious infections even be detected.

 HPV Test, HPV-DNA test, how is applied?

Mukussuz as bloodless and a vaginal smears taken easily with a brush or a small sample taken after the intervention condyloma HPV is a piece of him belongs to DNA to achieve (if it is positive HPV) is possible. Following the PCR results of DNA extraction followed by agarose gel imaging. Gel imaging (+) results of HPV DNA indicates the presence of them. Then, using chip technology for the most common type of examination is 35.

 HPV Test, HPV-DNA testing should be done to whom ?

- From the third year after the onset of active sexual life for the purpose of screening should be done and should be repeated once every three years.

Pap-smear-ASCUS / LSIL colposcopy after detection of the determination of indications,

Used to follow up after treatment of HPV-positivity.

 HPV material (smears) should be how?

Smear brush 5-6 times a clockwise direction and turning the swab is placed in the cervix, to be run as much as possible rash. Then smear brush bar should be left in a special solution and the molecular diagnostic center should be sent as soon as possible.

HPV Test, HPV DNA testing (-) predictive value of 99% is what makes sense?

HPV DNA test result (HPV test), negative (-) is 99% likely excludes the possibility of CIN2/CIN3, the presence of precancerous lesions in colposcopy evaluation and indications to provide very important information in determining the physician. The result is negative or low-risk types have been identified to be a lot of useless process eliminates the possibility.

 Pap smear and HPV-DNA test negative (-) of the people when a person should undergo screening?

ACS (American Cancer Society) and the ASCCP (American Society for Colposcopy andCervical Pathology) 's decision to partner with 3 years ago not seem necessary to repeat the Pap smear and HPV-DNA testing.

Pap smear negative (-), the HPV DNA positive (+) is what should be done?

After 6 months, according to ACS and ASCCP'e Pap smear and HPV DNA test should be repeated.

Six months later, the HPV DNA test (HPV test), again a positive (+) if what should be done?

According to the ACS and ASCCP'ye the result of the Pap smear negative (-), although HPV DNA testing positive (+) for colposcopy due to the specialist.

Pap smear-positive (+) and HPV-DNA positive (+) is what should be done?

ACS, ASCCP, and according to the direct colposcopy should be recommended.

Is there a relationship between the HPV virus in other cancers?

16 high-risk HPV genotypes anus, vulva, vagina and the penis was found to be associated with other anogenital cancers. In addition, some cancers, respiratory and gastrointestinal traktuslarda was also associated with HPV.

Why are screening tests should be made?

2 women's deaths from cancer when using the methods of early diagnosis of cervical cancer can take at an early stage. DNA-based tests with the use of screening tests have been taken seriously distances. Very easy to use these techniques can be applied to low-cost service can be given to preventive medicine and early detection, patients with serious, in terms of physicians and health care system is provided to all the material and spiritual benefits.

Are there other tests for the detection of HPV and innovations? What's New HPV Test?

HPV is a routine laboratory method for the culture was not developed yet. Many molecular methods can be used to detect HPV. These are the filter hybridization method (FISH), PCR, ligase chain reaction and direct cloning of HPV DNA. The FDA approval of a method other Vira Pap / Vira Type test. Last time, the Hybrid Capture test was introduced and received FDA approval.

"Micro array chip," "Method What is it?

More than 100 types of HPV are known to be 'the only 18'nin is dangerous, that can cause cancer are known. Regular smear tests, but 50 percent of the virus yakalanabilmekte, for accurate diagnosis, "micro-array chip" s necessary to use a lot of publications have been reported in recent years. This system is the most common types of HPV that cause cancer types and automatically identify and there is the possibility of error. Micro-array chip method can also be used in men, with more than 1 month 3 weeks ago in this system should have to wait a long time, the test results can be obtained within 4 days.